RARA (phospho S77) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated Jurkat cell lysates, Lane 2: Synthesized peptide treated Jurkat cell lysates reacted with RARA (phospho S77) polyclonal antibody (Cat # PAB29640) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue reacted with RARA (phospho S77) polyclonal antibody (Cat # PAB29640) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RARA.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S77 of human RARA.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human, Mouse, Rat
Specificity
RARA (phospho S77) polyclonal antibody detects endogenous levels of human RARA only when phosphorylated at serine 77.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated Jurkat cell lysates, Lane 2: Synthesized peptide treated Jurkat cell lysates reacted with RARA (phospho S77) polyclonal antibody (Cat # PAB29640) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue reacted with RARA (phospho S77) polyclonal antibody (Cat # PAB29640) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — RARA
Entrez GeneID
5914Protein Accession#
P10276Gene Name
RARA
Gene Alias
NR1B1, RAR
Gene Description
retinoic acid receptor, alpha
Omim ID
180240Gene Ontology
HyperlinkGene Summary
Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 17468032]).[supplied by OMIM
Other Designations
OTTHUMP00000164454|OTTHUMP00000164456|Retinoic acid receptor, alpha polypeptide|nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form
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