AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated K562 cell lysates, Lane 2: Synthesized peptide treated K562 cell lysates reacted with AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody (Cat # PAB29638) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody (Cat # PAB29638) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human AKT1/AKT3.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y437/Y434 of human AKT1/AKT3.
Host
Rabbit
Theoretical MW (kDa)
56
Reactivity
Human, Mouse, Rat
Specificity
AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody detects endogenous levels of human AKT1/AKT3 only when phosphorylated at tyrosine 437 and tyrosine 434.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated K562 cell lysates, Lane 2: Synthesized peptide treated K562 cell lysates reacted with AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody (Cat # PAB29638) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody (Cat # PAB29638) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749;Q9Y243Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Gene Info — AKT3
Entrez GeneID
10000Protein Accession#
P31749;Q9Y243Gene Name
AKT3
Gene Alias
DKFZp434N0250, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
Gene Description
v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Omim ID
611223Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000037911|OTTHUMP00000037912|RAC-gamma serine/threonine protein kinase|protein kinase B gamma|serine threonine protein kinase, Akt-3|v-akt murine thymoma viral oncogene homolog 3
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Interactome
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Pathway
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Disease
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