FMR1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of Lane 1: RT-4 cell lysate, Lane 2: U-251 MG cell lysate with FMR1 polyclonal antibody (Cat# PAB29497) at 1:250 - 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with FMR1 polyclonal antibody (Cat# PAB29497) shows strong cytoplasmic positivity in neuronal cells at 1:200 - 1:500 dilution.
Immunofluorescence
Immunofluorescent staining of U-251 MG cells with FMR1 polyclonal antibody (Cat# PAB29497) under 1-4 ug/mL working concentration shows positivity in cytoplasm. Antibody staining is shown in green.
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Specification
Product Description
FMR1 polyclonal antibody raised against recombinant human FMR1.
Immunogen
Recombinant protein corresponding to amino acids of human FMR1.
Sequence
FKGNDDHSRTDNRPRNPREAKGRTTDGSLQIRVDCNNERSVHTKTLQNTSSEGSRLRTGKDRNQKKEKPDSVDGQQPLVNGVP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of Lane 1: RT-4 cell lysate, Lane 2: U-251 MG cell lysate with FMR1 polyclonal antibody (Cat# PAB29497) at 1:250 - 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with FMR1 polyclonal antibody (Cat# PAB29497) shows strong cytoplasmic positivity in neuronal cells at 1:200 - 1:500 dilution.Immunofluorescence
Immunofluorescent staining of U-251 MG cells with FMR1 polyclonal antibody (Cat# PAB29497) under 1-4 ug/mL working concentration shows positivity in cytoplasm. Antibody staining is shown in green. -
Gene Info — FMR1
Entrez GeneID
2332Protein Accession#
Q06787Gene Name
FMR1
Gene Alias
FMRP, FRAXA, MGC87458, POF, POF1
Gene Description
fragile X mental retardation 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). [provided by RefSeq
Other Designations
OTTHUMP00000024197|premature ovarian failure 1
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Interactome
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Disease
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