ADAR polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG with ADAR polyclonal antibody (Cat # PAB29440) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with ADAR polyclonal antibody (Cat # PAB29440) shows strong nuclear positivity in neuronal cells at 1:1000-1:2500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ADAR polyclonal antibody (Cat # PAB29440) at 1-4 ug/mL concentration shows positivity in nucleoli and nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human ADAR.
Immunogen
Recombinant protein corresponding to human ADAR.
Sequence
ALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDY
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:1000-1:2500)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG with ADAR polyclonal antibody (Cat # PAB29440) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with ADAR polyclonal antibody (Cat # PAB29440) shows strong nuclear positivity in neuronal cells at 1:1000-1:2500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ADAR polyclonal antibody (Cat # PAB29440) at 1-4 ug/mL concentration shows positivity in nucleoli and nucleus but excluded from the nucleoli. -
Gene Info — ADAR
Entrez GeneID
103Gene Name
ADAR
Gene Alias
ADAR1, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88dsRBP, p136
Gene Description
adenosine deaminase, RNA-specific
Gene Ontology
HyperlinkGene Summary
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
136 kDa double-stranded RNA binding protein|OTTHUMP00000035372|OTTHUMP00000035373|adenosine deaminase acting on RNA 1-A|double-stranded RNA-specific adenosine deaminase|interferon-induced protein 4|interferon-inducible protein 4
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Interactome
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Pathway
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Disease
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