CCNC (phospho S275) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated 3T3 cells, Lane 2: 3T3 cells, Lane 3: A549 cells, Lane 4: Hela cells with CCNC (phospho S275) polyclonal antibody (Cat # PAB29241) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast carcinoma tissue by CCNC (phospho S275) polyclonal antibody (Cat # PAB29241) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CCNC.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S275 of human CCNC.
Host
Rabbit
Theoretical MW (kDa)
37
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of CCNC only when phosphorylated at serine 275.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated 3T3 cells, Lane 2: 3T3 cells, Lane 3: A549 cells, Lane 4: Hela cells with CCNC (phospho S275) polyclonal antibody (Cat # PAB29241) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast carcinoma tissue by CCNC (phospho S275) polyclonal antibody (Cat # PAB29241) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — CCNC
Entrez GeneID
892Protein Accession#
P24863Gene Name
CCNC
Gene Alias
CycC
Gene Description
cyclin C
Omim ID
123838Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000016897
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