LAP3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LAP3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human LAP3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LAP3 polyclonal antibody (Cat # PAB2871) in HepG2 cell line lysates (35 ug/lane). LAP3 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with LAP3 polyclonal antibody (Cat # PAB2871) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — LAP3
Entrez GeneID
51056Protein Accession#
NP_056991;P28838Gene Name
LAP3
Gene Alias
LAP, LAPEP, PEPS
Gene Description
leucine aminopeptidase 3
Omim ID
170250Gene Ontology
HyperlinkOther Designations
peptidase S
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Interactome
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Pathway
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Disease
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Publication Reference
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Reduced activity of the hypertension-associated Lys528Arg mutant of human adipocyte-derived leucine aminopeptidase (A-LAP)/ER-aminopeptidase-1.
Goto Y, Hattori A, Ishii Y, Tsujimoto M.
FEBS Letters 2006 Mar; 580(7):1833.
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Structural and immunological evidence for the identity of prolyl aminopeptidase with leucyl aminopeptidase.
Matsushima M, Takahashi T, Ichinose M, Miki K, Kurokawa K, Takahashi K.
Biochemical and Biophysical Research Communications 1991 Aug; 178(3):1459.
Application:Enzyme, Recombinant proteins.
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Reduced activity of the hypertension-associated Lys528Arg mutant of human adipocyte-derived leucine aminopeptidase (A-LAP)/ER-aminopeptidase-1.
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