HNF1B polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using HNF1B polyclonal antibody (Cat # PAB28567).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Immunofluorescence
Immunofluorescence of U-251 MG cell line with HNF1B polyclonal antibody (Cat # PAB28567) shows positivity in nucleus but not nucleoli. Fixation/Permeabilization: PFA/Triton X-100
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant HNF1B.
Immunogen
Recombinant protein corresponding to human HNF1B.
Sequence
KEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQH
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:250-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using HNF1B polyclonal antibody (Cat # PAB28567).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)Immunofluorescence
Immunofluorescence of U-251 MG cell line with HNF1B polyclonal antibody (Cat # PAB28567) shows positivity in nucleus but not nucleoli. Fixation/Permeabilization: PFA/Triton X-100 -
Gene Info — HNF1B
Entrez GeneID
6928Protein Accession#
E0YMJ9Gene Name
HNF1B
Gene Alias
FJHN, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, TCF2, VHNF1
Gene Description
HNF1 homeobox B
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
transcription factor 2|transcription factor 2, hepatic|transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor
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Interactome
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Pathway
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Disease
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