CKM polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
CKM polycolnal antibody (Cat # PAB27681) (0.03ug/ml) staining of Lane 1: human and Lane 2: mouse heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CKM.
Immunogen
A synthetic peptide corresponding to amino acids 258-270 at internal region of human CKM.
Sequence
QKIEEIFKKAGHP
Host
Goat
Theoretical MW (kDa)
45
Reactivity
Chicken, Dog, Human, Mouse, Pig, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.03-0.1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CKM polycolnal antibody (Cat # PAB27681) (0.03ug/ml) staining of Lane 1: human and Lane 2: mouse heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — CKM
Entrez GeneID
1158Protein Accession#
NP_001815.2Gene Name
CKM
Gene Alias
CKMM, M-CK
Gene Description
creatine kinase, muscle
Omim ID
123310Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq
Other Designations
creatine kinase M chain|creatine kinase-M|muscle creatine kinase
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Interactome
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Pathway
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Disease
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