RNF20 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RNF20.
Immunogen
A synthetic peptide corresponding to 19 amino acids at N-terminus of human RNF20.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
Multiple isoforms of RNF20 are known to exist.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (2.5 ug/mL)
Immunofluorescence (20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of RNF20 in human liver tissue with RNF20 polyclonal antibody (Cat # PAB27430) at (lane 1) 1 and (lane 2) 2 ug/mL.Immunohistochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF20
Entrez GeneID
56254Protein Accession#
NP_062538Gene Name
RNF20
Gene Alias
BRE1, BRE1A, FLJ11189, FLJ20382, KIAA2779, MGC129667, MGC129668, hBRE1
Gene Description
ring finger protein 20
Omim ID
607699Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3. [provided by RefSeq
Other Designations
BRE1 E3 ubiquitin ligase homolog|homolog of S. cerevisiae BRE1
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