IDO1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of IDO1 polyclonal antibody (Cat # PAB2571) in 293 cell line lysates (35 ug/lane). IDO1 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinomareacted with IDO1 polyclonal antibody (Cat # PAB2571), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IDO1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human IDO1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemsitry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of IDO1 polyclonal antibody (Cat # PAB2571) in 293 cell line lysates (35 ug/lane). IDO1 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinomareacted with IDO1 polyclonal antibody (Cat # PAB2571), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — IDO1
Entrez GeneID
3620Protein Accession#
NP_002155;P14902Gene Name
IDO1
Gene Alias
CD107B, IDO, INDO
Gene Description
indoleamine 2,3-dioxygenase 1
Omim ID
147435Gene Ontology
HyperlinkGene Summary
Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.[supplied by OMIM
Other Designations
indole 2,3-dioxygenase|indoleamine-pyrrole 2,3 dioxygenase
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Interactome
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Pathway
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Disease
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Publication Reference
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The role of serine 167 in human indoleamine 2,3-dioxygenase: a comparison with tryptophan 2,3-dioxygenase.
Chauhan N, Basran J, Efimov I, Svistunenko DA, Seward HE, Moody PC, Raven EL.
Biochemistry 2008 Apr; 47(16):4761.
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Cytochrome b5, not superoxide anion radical, is a major reductant of indoleamine 2,3-dioxygenase in human cells.
Maghzal GJ, Thomas SR, Hunt NH, Stocker R.
The Journal of Biological Chemistry 2008 May; 283(18):12014.
Application：WB-Ce, WB-Tr, Human, Human aortic endothelial cells, HEK 293 cells.
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Indoleamine 2,3-dioxygenase (IDO): the antagonist of type I interferon-driven skin inflammation?
Scheler M, Wenzel J, Tuting T, Takikawa O, Bieber T, von Bubnoff D.
The American Journal of Pathology 2007 Dec; 171(6):1936.
Application：IF, IHC-P, Human, Human skin biopsies.
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The role of serine 167 in human indoleamine 2,3-dioxygenase: a comparison with tryptophan 2,3-dioxygenase.
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