OLFM1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of OLFM1 (arrow) using rabbit OLFM1 polyclonal antibody (Cat # PAB2558). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OLFM1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with OLFM1 polyclonal antibody (Cat # PAB2558) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of OLFM1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human OLFM1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of OLFM1 (arrow) using rabbit OLFM1 polyclonal antibody (Cat # PAB2558). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OLFM1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with OLFM1 polyclonal antibody (Cat # PAB2558) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — OLFM1
Entrez GeneID
10439Protein Accession#
NP_006325;Q99784Gene Name
OLFM1
Gene Alias
AMY, NOE1, NOELIN1, OlfA
Gene Description
olfactomedin 1
Omim ID
605366Gene Ontology
HyperlinkGene Summary
This gene product shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of the encoded protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue. Several alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000022526|OTTHUMP00000022527|neuroblastoma protein|noelin|olfactomedin related ER localized protein|pancortin 1
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Interactome
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Disease
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Publication Reference
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Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.
Liu T, Qian WJ, Gritsenko MA, Camp DG 2nd, Monroe ME, Moore RJ, Smith RD.
Journal of Proteome Research 2005 Nov; 4(6):2070.
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Large-scale concatenation cDNA sequencing.
Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA.
Genome Research 1997 Apr; 7(4):353.
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Identification and cloning of neuroblastoma-specific and nerve tissue-specific genes through compiled expression profiles.
Yokoyama M, Nishi Y, Yoshii J, Okubo K, Matsubara K.
DNA Research 1996 Oct; 3(5):311.
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Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.
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