KIF25 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with KIF25 polyclonal antibody (Cat # PAB24475).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with KIF25 polyclonal antibody (Cat # PAB24475) shows strong cytoplasmic positivity in glandular cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant KIF25.
Immunogen
Recombinant protein corresponding to amino acids of human KIF25.
Sequence
ITVTLTTASCSDSTADQACSATLPREQTEAGRAGRSRRASQGALAPQLVPGNPAGHAEQVQARLQLVDS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with KIF25 polyclonal antibody (Cat # PAB24475).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with KIF25 polyclonal antibody (Cat # PAB24475) shows strong cytoplasmic positivity in glandular cells. -
Gene Info — KIF25
Entrez GeneID
3834Protein Accession#
Q9UIL4Gene Name
KIF25
Gene Alias
KNSL3, MGC163361
Gene Description
kinesin family member 25
Omim ID
603815Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. However, the particular function of this gene product has not yet been determined. Two alternatively spliced transcript variants which encode products have been described. Other splice variants have been found that lack exon 2 and the initiation codon for translation. [provided by RefSeq
Other Designations
OTTHUMP00000017650|kinesin-like 3
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