Products

Western Blot (Transfected lysate)

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with DDX23 polyclonal antibody (Cat # PAB23506) at 1:250-1:500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining of human prostate with DDX23 polyclonal antibody (Cat # PAB23506) shows strong nuclear positivity in glandular cells at 1:1000-1:2500 dilution.

Immunofluorescence

Immunofluorescent staining of human cell line U-251 MG with DDX23 polyclonal antibody (Cat # PAB23506) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and plasma membrane.

Entrez GeneID

9416

Protein Accession#

Q9BUQ8

Gene Name

DDX23

Gene Alias

MGC8416, PRPF28, U5-100K, U5-100KD, prp28

Gene Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 23

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq

Other Designations

PRP28 homolog, yeast|PRP28p homolog|U5 snRNP 100 kD protein