PAK4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The PAK4 polyclonal antibody (Cat # PAB2301) is used in Western blot to detect PAK4 in mouse small intestine tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the PAK4 polyclonal antibody (Cat # PAB2301) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PAK4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PAK4.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PAK4 polyclonal antibody (Cat # PAB2301) is used in Western blot to detect PAK4 in mouse small intestine tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the PAK4 polyclonal antibody (Cat # PAB2301) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — PAK4
Entrez GeneID
10298Protein Accession#
O96013Gene Name
PAK4
Gene Alias
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Gene Description
p21 protein (Cdc42/Rac)-activated kinase 4
Omim ID
605451Gene Ontology
HyperlinkGene Summary
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
p21(CDKN1A)-activated kinase 4|p21-activated kinase 4|protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
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Interactome
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Pathway
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Disease
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Publication Reference
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p21-activated protein kinase 4 (PAK4) interacts with the keratinocyte growth factor receptor and participates in keratinocyte growth factor-mediated inhibition of oxidant-induced cell death.
Lu Y, Pan ZZ, Devaux Y, Ray P.
The Journal of Biological Chemistry 2003 Mar; 278(12):10374.
Application：WB-Ce, Human, HEK 293 cells.
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Pak to the future.
Bagrodia S, Cerione RA.
Trends in Cell Biology 1999 Sep; 9(9):350.
Application：IHC, WB-Ce, WB-Tr, Human, Cancers, Mammalian cells.
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PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia.
Abo A, Qu J, Cammarano MS, Dan C, Fritsch A, Baud V, Belisle B, Minden A.
The EMBO Journal 1998 Nov; 17(22):6527.
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p21-activated protein kinase 4 (PAK4) interacts with the keratinocyte growth factor receptor and participates in keratinocyte growth factor-mediated inhibition of oxidant-induced cell death.
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