EN1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of EN1 polyclonal antibody (Cat # PAB2058) in NCI-H460 cell line lysates (35 ug/lane). EN1 (arrow) was detected using the purified Polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 polyclonal antibody (Cat # PAB2058) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EN1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EN1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EN1 polyclonal antibody (Cat # PAB2058) in NCI-H460 cell line lysates (35 ug/lane). EN1 (arrow) was detected using the purified Polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 polyclonal antibody (Cat # PAB2058) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — EN1
Entrez GeneID
2019Protein Accession#
NP_001417;Q05925Gene Name
EN1
Gene Alias
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Gene Description
engrailed homeobox 1
Omim ID
131290Gene Ontology
HyperlinkGene Summary
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq
Other Designations
OTTHUMP00000162091|engrailed homolog 1
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Interactome
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Disease
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Publication Reference
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Engrailed-1 negatively regulates beta-catenin transcriptional activity by destabilizing beta-catenin via a glycogen synthase kinase-3beta-independent pathway.
Bachar-Dahan L, Goltzmann J, Yaniv A, Gazit A.
Molecular Biology of the Cell 2006 Jun; 17(6):2572.
Application：WB-Tr, Human, HEK 293T cells.
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Regional assignment of the human homeobox-containing gene EN1 to chromosome 2q13-q21.
Kohler A, Logan C, Joyner AL, Muenke M.
Genomics 1993 Jan; 15(1):233.
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Engrailed-1 negatively regulates beta-catenin transcriptional activity by destabilizing beta-catenin via a glycogen synthase kinase-3beta-independent pathway.
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