ME2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ME2.
Immunogen
Recombinant protein corresponding to amino acids of human ME2.
Sequence
KVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTA
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ME2 polyclonal antibody (Cat # PAB20457) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum with ME2 polyclonal antibody (Cat # PAB20457) shows strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ME2 polyclonal antibody (Cat # PAB20457) at 1-4 ug/mL dilution shows positivity in mitochondria. -
Gene Info — ME2
Entrez GeneID
4200Protein Accession#
P23368Gene Name
ME2
Gene Alias
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Gene Description
malic enzyme 2, NAD(+)-dependent, mitochondrial
Gene Ontology
HyperlinkGene Summary
This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. [provided by RefSeq
Other Designations
Malic enzyme, mitochondrial|malate dehydrogenase|malic enzyme 2, mitochondrial|pyruvic-malic carboxylase
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Interactome
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Pathway
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Disease
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