GRHL1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant GRHL1.
Immunogen
Recombinant protein corresponding to amino acids of human GRHL1.
Sequence
TVFKPFIDLDTQPVLFIPDVHFANLQRGTHVLPIASEELEGEGSVLKRGPYGTEDDFAVPPSTKLARIEEPKRVLLYVRKESEEVFDALMLKTPSLKGL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates with GRHL1 polyclonal antibody (Cat # PAB20320) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-IIWestern Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with GRHL1 polyclonal antibody (Cat # PAB20320) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human esophagus with GRHL1 polyclonal antibody (Cat # PAB20320) shows strong nuclear positivity in squamous epithelial cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with GRHL1 polyclonal antibody (Cat # PAB20320) at 1-4 ug/mL dilution shows in nucleus, golgi apparatus. -
Gene Info — GRHL1
Entrez GeneID
29841Protein Accession#
Q9NZI5Gene Name
GRHL1
Gene Alias
LBP-32, LBP32, MGR, TFCP2L2
Gene Description
grainyhead-like 1 (Drosophila)
Omim ID
609786Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the grainyhead family of transcription factors. The encoded protein can exist as a homodimer or can form heterodimers with sister-of-mammalian grainyhead or brother-of-mammalian grainyhead. This protein functions as a transcription factor during development. [provided by RefSeq
Other Designations
LBP protein 32|leader-binding protein 32|mammalian grainyhead|transcription factor CP2-like 2
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