HICE1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of S phase HeLa cells with HICE1 polyclonal antibody (Cat # PAB19929) at 1:10000 dilution. Lane 1: lysates from sh-Luciferase lentivirus-infected cells as control; Lane 2: lysates from sh-HICE1 RNAi-treated lentivirus-infected cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant HICE1.
Immunogen
Recombinant GST fusion protein corresponding to full length human HICE1.
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Human
Specificity
This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence.
Form
Liquid
Purification
Immunoaffinity purification
Recommend Usage
ELISA (1:250000)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of S phase HeLa cells with HICE1 polyclonal antibody (Cat # PAB19929) at 1:10000 dilution. Lane 1: lysates from sh-Luciferase lentivirus-infected cells as control; Lane 2: lysates from sh-HICE1 RNAi-treated lentivirus-infected cells.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HICE1
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Interactome
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