DEFA1/DEFA3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
DEFA1|DEFA3 polyclonal antibody (Cat # PAB18743) (2 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DEFA1|DEFA3 polyclonal antibody (Cat # PAB18743) (3.8 ug/mL) staining of paraffin embedded human thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DEFA1/DEFA3.
Immunogen
A synthetic peptide corresponding to internal region of human DEFA1/DEFA3.
Sequence
C-DESLAPKHPGSRKN
Host
Goat
Theoretical MW (kDa)
10.2
Reactivity
Human
Specificity
This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DEFA1|DEFA3 polyclonal antibody (Cat # PAB18743) (2 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DEFA1|DEFA3 polyclonal antibody (Cat # PAB18743) (3.8 ug/mL) staining of paraffin embedded human thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DEFA1
Entrez GeneID
1667Protein Accession#
NP_004075.1 (Gene ID : 1667);NP_005208.1 (Gene ID : 1668)Gene Name
DEFA1
Gene Alias
DEF1, DEFA2, HNP-1, HP-1, MGC138393, MRS
Gene Description
defensin, alpha 1
Omim ID
125220Gene Ontology
HyperlinkGene Summary
Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. It differs from defensin, alpha 3 by only one amino acid. [provided by RefSeq
Other Designations
OTTHUMP00000196017|defensin, alpha 1, myeloid-related sequence|defensin, alpha 2|myeloid-related sequence
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Gene Info — DEFA3
Entrez GeneID
1668Protein Accession#
NP_004075.1 (Gene ID : 1667);NP_005208.1 (Gene ID : 1668)Gene Name
DEFA3
Gene Alias
DEF3, HNP-3, HNP3, HP-3
Gene Description
defensin, alpha 3, neutrophil-specific
Omim ID
604522Gene Ontology
HyperlinkGene Summary
Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. It differs from defensin, alpha 1 by only one amino acid. [provided by RefSeq
Other Designations
defensin 3, neutrophil-specific|defensin, alpha 3|neutrophil peptide 3
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