ATP1B1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ATP1B1 polyclonal antibody (Cat # PAB18731) (0.1 ug/mL) staining of rat retina lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ATP1B1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human ATP1B1.
Sequence
C-KTEISFRPNDPKSYE
Host
Goat
Theoretical MW (kDa)
48
Reactivity
Mouse, Rat
Specificity
This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ATP1B1 polyclonal antibody (Cat # PAB18731) (0.1 ug/mL) staining of rat retina lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATP1B1
Entrez GeneID
481Protein Accession#
NP_001668.1;NP_001001787.1Gene Name
ATP1B1
Gene Alias
ATP1B, MGC1798
Gene Description
ATPase, Na+/K+ transporting, beta 1 polypeptide
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
Beta 1-subunit of Na(+),K(+)-ATPase|Na+/K+ -ATPase beta 1 subunit|Na, K-ATPase beta-1 polypeptide|Na,K-ATPase beta 1 subunit|OTTHUMP00000032537|OTTHUMP00000032538|adenosinetriphosphatase|sodium/potassium-dependent ATPase beta-1 subunit|sodium/potassium-tr
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Interactome
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Pathway
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Disease
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