MAP3K6 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
MAP3K6 polyclonal antibody (Cat # PAB17236) (0.3 ug/mL) staining of rat heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MAP3K6.
Immunogen
A synthetic peptide corresponding to human MAP3K6.
Sequence
C-RAILAQRAGSTP
Host
Goat
Theoretical MW (kDa)
143
Reactivity
Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MAP3K6 polyclonal antibody (Cat # PAB17236) (0.3 ug/mL) staining of rat heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAP3K6
Entrez GeneID
9064Protein Accession#
NP_004663.3Gene Name
MAP3K6
Gene Alias
ASK2, MAPKKK6, MGC125653, MGC20114
Gene Description
mitogen-activated protein kinase kinase kinase 6
Omim ID
604468Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000005063|apoptosis signal-regulating kinase 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Mitogen-activated protein 3 kinase 6 mediates angiogenic and tumorigenic effects via vascular endothelial growth factor expression.
Eto N, Miyagishi M, Inagi R, Fujita T, Nangaku M.
The American Journal of Pathology 2009 Apr; 174(4):1553.
Application：WB-Tr, Human, HeLa S3 cells.
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Mitogen-activated protein 3 kinase 6 mediates angiogenic and tumorigenic effects via vascular endothelial growth factor expression.
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