IKK-alpha/beta (phospho S176) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of IKK-alpha/beta.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S176 of human IKK-alpha/beta.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of IKK-alpha/IKK-beta only when phosphorylated at serine 180/181.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IKKα/IKKβ (phospho Ser176) polyclonal antibody (Cat # PAB16976).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Proteasome inhibitors induce death but activate NF-kappaB on endometrial carcinoma cell lines and primary culture explants.
Dolcet X, Llobet D, Encinas M, Pallares J, Cabero A, Schoenenberger JA, Comella JX, Matias-Guiu X.
The Journal of Biological Chemistry 2006 May; 281(31):22118.
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NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176.
Ling L, Cao Z, Goeddel DV.
PNAS 1998 Mar; 95(7):3792.
Application:WB-Tr, Human, HEK 293, HeLa cells.
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IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation.
Mercurio F, Zhu H, Murray BW, Shevchenko A, Bennett BL, Li J, Young DB, Barbosa M, Mann M, Manning A, Rao A.
Science 1997 Oct; 278(5339):860.
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Proteasome inhibitors induce death but activate NF-kappaB on endometrial carcinoma cell lines and primary culture explants.
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