Ccl20 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using Ccl20 polyclonal antibody (Cat # PAB16930) shows detection of a band ~11 KDa in size corresponding to recombinant mouse Ccl20. Recombinant mouse Ccl20 was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Ccl20.
Immunogen
Recombinant protein corresponding to full length mouse Ccl20.
Host
Rabbit
Reactivity
Mouse, Rat
Form
Lyophilized
Recommend Usage
ELISA (1:10000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using Ccl20 polyclonal antibody (Cat # PAB16930) shows detection of a band ~11 KDa in size corresponding to recombinant mouse Ccl20. Recombinant mouse Ccl20 was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ccl20
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Publication Reference
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C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE.
Reboldi A, Coisne C, Baumjohann D, Benvenuto F, Bottinelli D, Lira S, Uccelli A, Lanzavecchia A, Engelhardt B, Sallusto F.
Nature Immunology 2009 Mar; 10(5):514.
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Toll-like receptor 5- and lymphotoxin beta receptor-dependent epithelial Ccl20 expression involves the same NF-kappaB binding site but distinct NF-kappaB pathways and dynamics.
Sirard JC, Didierlaurent A, Cayet D, Sierro F, Rumbo M.
Biochimica et Biophysica Acta 2009 Mar; 1789(5):386.
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Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis.
Harper EG, Guo C, Rizzo H, Lillis JV, Kurtz SE, Skorcheva I, Purdy D, Fitch E, Iordanov M, Blauvelt A.
The Journal of Investigative Dermatology 2009 Mar; 129(9):2175.
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C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE.
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