CCL2 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using CCL2 polyclonal antibody (Cat # PAB16914) shows detection of recombinant bovine CCL2 at 8.8 KDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with CCL2 polyclonal antibody. After washing, membrane was probed with Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant CCL2.
Immunogen
Recombinant protein corresponding to 76 amino acids of bovine CCL2.
Host
Rabbit
Reactivity
Bovine
Form
Lyophilized
Recommend Usage
ELISA (1:4500)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using CCL2 polyclonal antibody (Cat # PAB16914) shows detection of recombinant bovine CCL2 at 8.8 KDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with CCL2 polyclonal antibody. After washing, membrane was probed with Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCL2
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Publication Reference
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Short communication: Localization and expression of monocyte chemoattractant protein-1 in different subcutaneous and visceral adipose tissues of early-lactating dairy cows.
Haussler S, Sacre C, Friedauer K, Danicke S, Sauerwein H.
Journal of Dairy Science 2015 Sep; 98(9):6278.
Application：IHC, Cow, Adipose tissue.
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CCL2 (Monocyte Chemoattractant Protein-1) in cancer bone metastases.
Craig MJ, Loberg RD.
Cancer Metastasis Reviews 2006 Dec; 25(4):611.
Application：ELISA, Human, C4-2B, LNCaP, PC-3, VCaP cells, Human colorectal carcinoma, Normal mucosa.
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Cooperative expression of monocyte chemoattractant protein 1 within the bovine corpus luteum: evidence of immune cell-endothelial cell interactions in a coculture system.
Liptak AR, Sullivan BT, Henkes LE, Wijayagunawardane MP, Miyamoto A, Davis JS, Rueda BR, Townson DH.
Biology of Reproduction 2005 May; 72(5):1169.
Application：ELISA, Bovine, Culture medium from endothelial, peripheral blood mononuclear cells.
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Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro.
Xu LL, Warren MK, Rose WL, Gong W, Wang JM.
Journal of Leukocyte Biology 1996 Sep; 60(3):365.
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Short communication: Localization and expression of monocyte chemoattractant protein-1 in different subcutaneous and visceral adipose tissues of early-lactating dairy cows.
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