PRDM1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blots using PRDM1 polyclonal antibody (Cat # PAB16909) show detection of overexpressed PRDM1 in whole transfected Raji cell lysate (Lane 2) at ~88kDa. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1 (Lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (Lane 3). The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1 : 1000 dilution in 5% PBS-Tween.
Personal communication, Hyesuk Yoon and Jerry Boss, Emory University, Atlanta, GA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRDM1.
Immunogen
A synthetic peptide corresponding to C-terminus of human PRDM1 .
Host
Rabbit
Reactivity
Dog, Human, Mouse, Rat
Form
Liquid
Recommend Usage
ELISA (1:65000-1:80000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blots using PRDM1 polyclonal antibody (Cat # PAB16909) show detection of overexpressed PRDM1 in whole transfected Raji cell lysate (Lane 2) at ~88kDa. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1 (Lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (Lane 3). The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1 : 1000 dilution in 5% PBS-Tween.
Personal communication, Hyesuk Yoon and Jerry Boss, Emory University, Atlanta, GA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRDM1
Entrez GeneID
639Gene Name
PRDM1
Gene Alias
BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1
Gene Description
PR domain containing 1, with ZNF domain
Omim ID
603423Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq
Other Designations
B-lymphocyte-induced maturation protein 1|OTTHUMP00000016918|PR-domain zinc finger protein 1|PRDI-binding factor-1|beta-interferon gene positive-regulatory domain I binding factor|positive regulatory domain I-binding factor 1
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Interactome
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Disease
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Publication Reference
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MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation.
Fujita N, Jaye DL, Geigerman C, Akyildiz A, Mooney MR, Boss JM, Wade PA.
Cell 2004 Oct; 119(1):75.
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Kinetics of a gamma interferon response: expression and assembly of CIITA promoter IV and inhibition by methylation.
Morris AC, Beresford GW, Mooney MR, Boss JM.
Molecular and Cellular Biology 2002 Jul; 22(13):4781.
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Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells.
Nagy M, Chapuis B, Matthes T.
British Journal of Haematology 2002 Feb; 116(2):429.
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MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation.
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