FBXO32 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
FBXO32 polyclonal antibody (Cat # PAB15627) (0.1 ug/mL) staining of human skeletal muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of FBXO32.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human FBXO32.
Sequence
C-NSKTKTQYFHQEK
Host
Goat
Theoretical MW (kDa)
41.6, 24.7
Reactivity
Human, Mouse
Specificity
Approximately 40 KDa band observed in human and mouse skeletal muscle lysates (calculated MW of 41.6 KDa according to human NP_478136.1 and of 41.5 KDa according to mouse NP_080622.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FBXO32 polyclonal antibody (Cat # PAB15627) (0.1 ug/mL) staining of human skeletal muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FBXO32
Entrez GeneID
114907Protein Accession#
NP_478136.1Gene Name
FBXO32
Gene Alias
FLJ32424, Fbx32, MAFbx, MGC33610
Gene Description
F-box protein 32
Omim ID
606604Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing of this gene results in two transcript variants encoding two isoforms of different sizes. [provided by RefSeq
Other Designations
F-box only protein 32|atrogin 1|muscle atrophy F-box protein
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Interactome
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Publication Reference
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Muscle-specific MicroRNA1 (miR1) Targets Heat Shock Protein 70 (HSP70) during Dexamethasone-mediated Atrophy.
Kukreti H, Amuthavalli K, Harikumar A, Sathiyamoorthy S, Feng PZ, Anantharaj R, Tan SL, Lokireddy S, Bonala S, Sriram S, McFarlane C, Kambadur R, Sharma M.
The Journal of Biological Chemistry 2013 Mar; 288(9):6663.
Application：WB, Mouse, C2C12 cells.
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The muscle-specific ubiquitin ligase atrogin-1/MAFbx mediates statin-induced muscle toxicity.
Hanai J, Cao P, Tanksale P, Imamura S, Koshimizu E, Zhao J, Kishi S, Yamashita M, Phillips PS, Sukhatme VP, Lecker SH.
The Journal of Clinical Investigation 2007 Dec; 117(12):3940.
Application：WB-Ce, WB-Ti, Fish, Mouse, C2C12 cells, Fish embryos.
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Muscle-specific MicroRNA1 (miR1) Targets Heat Shock Protein 70 (HSP70) during Dexamethasone-mediated Atrophy.
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