CYBB polyclonal antibody
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More Files
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Western Blot (Cell lysate)
CYBB polyclonal antibody (Cat # PAB14389) (0.03 ug/mL) staining of human peripheral blood mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CYBB.
Immunogen
A synthetic peptide corresponding to human CYBB.
Sequence
C-SYLNFARKRIKNP
Host
Goat
Theoretical MW (kDa)
65.3
Reactivity
Human
Specificity
Approx 85 KDa band observed in human peripheral blood mononucleocytes ysates (calculated MW of 65.3KDa according to NP_000388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Teahan et al, Nature. 1987 Jun 25-Jul 1;327(6124):720-1. PMID: 3600769).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CYBB polyclonal antibody (Cat # PAB14389) (0.03 ug/mL) staining of human peripheral blood mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CYBB
Entrez GeneID
1536Protein Accession#
NP_000388.2Gene Name
CYBB
Gene Alias
CGD, GP91-1, GP91-PHOX, GP91PHOX, NOX2, p91-PHOX
Gene Description
cytochrome b-245, beta polypeptide
Gene Ontology
HyperlinkGene Summary
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq
Other Designations
OTTHUMP00000031915|cytochrome b-245 beta polypeptide
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Interactome
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Pathway
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Disease
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Publication Reference
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Constitutive activation of SHP2 protein tyrosine phosphatase inhibits ICSBP-induced transcription of the gene encoding gp91PHOX during myeloid differentiation.
Zhu C, Lindsey S, Konieczna I, Eklund EA.
Journal of Leukocyte Biology 2008 Mar; 83(3):680.
Application：WB-Ce, Mouse, Mouse bone marrow cells.
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Constitutive activation of SHP2 protein tyrosine phosphatase inhibits ICSBP-induced transcription of the gene encoding gp91PHOX during myeloid differentiation.
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