TRIM5-alpha polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TRIM5.
Immunogen
A synthetic peptide corresponding to C-terminus of monkey TRIM5.
Host
Rabbit
Reactivity
Human, Monkey
Specificity
A band at approximately 55 KDa can be detected. Anti-TRIM5 alpha is human specific but should presumably also react with rhesus protein.
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of TRIM5 expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha polyclonal antibody (Cat # PAB13409) at 2 ug /mL .Immunofluorescence
Immunofluorescence of TRIM5 alpha in human breast tissue with TRIM5 alpha polyclonal antibody (Cat # PAB13409) at 10 ug/mL . -
Gene Info — TRIM5
Entrez GeneID
574288Protein Accession#
NP_149023 (Human)Gene Name
TRIM5
Gene Alias
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Gene Description
tripartite motif-containing 5
Gene Ontology
HyperlinkOther Designations
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Publication Reference
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Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha.
Hatziioannou T, Perez-Caballero D, Yang A, Cowan S, Bieniasz PD.
PNAS 2004 Jul; 101(29):10774.
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The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys.
Stremlau M, Owens CM, Perron MJ, Kiessling M, Autissier P, Sodroski J.
Nature 2004 Feb; 427(6977):848.
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The tripartite motif family identifies cell compartments.
Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A.
The EMBO Journal 2001 May; 20(9):2140.
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Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha.
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