MED28 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of MED28 in human brain tissue lysate with MED28 polyclonal antibody (Cat # PAB13054) at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry
Immunohistochemical staining of human brain tissue with 2.5 ug/mL MED28 polyclonal antibody (Cat # PAB13054).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MED28.
Immunogen
A synthetic peptide corresponding to internal region 17 amino acids of human MED28.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of MED28 in human brain tissue lysate with MED28 polyclonal antibody (Cat # PAB13054) at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.Immunohistochemistry
Immunohistochemical staining of human brain tissue with 2.5 ug/mL MED28 polyclonal antibody (Cat # PAB13054). -
Gene Info — MED28
Entrez GeneID
80306Protein Accession#
NP_079481Gene Name
MED28
Gene Alias
1500003D12Rik, DKFZp434N185, EG1, magicin
Gene Description
mediator complex subunit 28
Omim ID
610311Gene Ontology
HyperlinkGene Summary
subunit 28 homolog
Other Designations
endothelial-derived gene 1|mediator of RNA polymerase II transcription, subunit 28 homolog
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Interactome
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Disease
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Publication Reference
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Identification of host proteins required for HIV infection through a functional genomic screen.
Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ.
Science 2008 Jan; 319(5865):921.
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Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation.
Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V.
The Journal of Biological Chemistry 2007 Nov; 282(44):32152.
Application：WB-Tr, Mouse, C2C12, NIH3T3 cells.
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A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC.
Molecular Cell 2004 Jun; 14(5):685.
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Identification of host proteins required for HIV infection through a functional genomic screen.
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