TNNT2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Tissue lysate from mouse heart was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by TNNT2 polyclonal antibody (Cat # PAB12744) at 1 : 500, right lane. Left lane was depleted by immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cardiac muscle stained with TNNT2 polyclonal antibody (Cat # PAB12744) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TNNT2.
Immunogen
A synthetic peptide corresponding to internal domain of human TNNT2.
Host
Rabbit
Theoretical MW (kDa)
31
Reactivity
Cat, Dog, Human, Mouse, Rabbit
Specificity
This antibody recognizes 31 KDa of TNNT2 by Western blot.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Tissue lysate from mouse heart was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by TNNT2 polyclonal antibody (Cat # PAB12744) at 1 : 500, right lane. Left lane was depleted by immunizing peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cardiac muscle stained with TNNT2 polyclonal antibody (Cat # PAB12744) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — TNNT2
Entrez GeneID
7139Gene Name
TNNT2
Gene Alias
CMH2, CMPD2, MGC3889, RCM3, TnTC, cTnT
Gene Description
troponin T type 2 (cardiac)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq
Other Designations
OTTHUMP00000033864|OTTHUMP00000033867|cardiomyopathy, dilated 1D (autosomal dominant)|cardiomyopathy, hypertrophic 2|troponin T type 2, cardiac|troponin T, cardiac muscle|troponin T2, cardiac
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Interactome
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Pathway
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Disease
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