ESR1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ESR1.
Immunogen
A synthetic peptide corresponding to residues surrounding S305 of human ESR1.
Host
Rabbit
Theoretical MW (kDa)
67
Reactivity
Human
Specificity
This antibody recognizes ~67 KDa of ESR1 without phosphorylation site of SESR1 305 (It is confirmed by dot blot). It does not cross react to phosphorylation site of SESR1305 of ESR1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysates derived from MCF-7 were immunoblotted by ESR1 polyclonal antibody (Cat # PAB12620) at 1 : 500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (Lane 2).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ESR1
Entrez GeneID
2099Gene Name
ESR1
Gene Alias
DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1
Gene Description
estrogen receptor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq
Other Designations
OTTHUMP00000017718|OTTHUMP00000017719|estrogen receptor alpha
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Interactome
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Disease
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Publication Reference
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TFAP2C regulates carbonic anhydrase XII in human breast cancer.
Franke CM, Gu VW, Grimm BG, Cassady VC, White JR, Weigel RJ, Kulak MV.
Oncogene 2020 Feb; 39(6):1290.
Application:ChIP, Human, BT-474, MCF-7, T47-D, MDA-MB-453, SKBR-3 cells.
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Gender matters: estrogen receptor alpha (ERα) and histone deacetylase (HDAC) 1 and 2 control the gender-specific transcriptional regulation of human uridine diphosphate glucuronosyltransferases genes (UGT1A).
Kalthoff S, Winkler A, Freiberg N, Manns MP, Strassburg CP.
Journal of Hepatology 2013 Oct; 59(4):797.
Application:ChIP, Human, SW403, KYSE70 cells.
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Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains.
Dutertre M, Smith CL.
Molecular Endocrinology (Baltimore, Md.) 2003 Apr; 17(7):1296.
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TFAP2C regulates carbonic anhydrase XII in human breast cancer.
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