ESR1(Phospho S118) recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ESR1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against KLH conjugated Synthesised phosphopeptide derived from human ESR1 around the phosphorylation site of Ser118.
Theoretical MW (kDa)
66
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of Estrogen Receptor alpha protein only when phosphorylated at Ser118.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.01M TBS, pH7.4 (1% BSA, 0.03% Proclin300 and 50% Glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded human breast using anti using ESR1(Phospho S118) recombinant monoclonal antibody (Cat # RAB02633). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C. -
Gene Info — ESR1
Entrez GeneID
2099Protein Accession#
P03372Gene Name
ESR1
Gene Alias
DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1
Gene Description
estrogen receptor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq
Other Designations
OTTHUMP00000017718|OTTHUMP00000017719|estrogen receptor alpha
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