ENO2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of ENO2 in rat spinal cord probed with ENO2 polyclonal antibodyCat # PAB12508 ). The antibody stains a single sharp band corresponing to NSE at about 47kDa.
Immunofluorescence
The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to ENO2 using ENO2 polyclonal antibody (Cat # PAB12508). This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant ENO2.
Immunogen
Recombinant protein corresponding to full length human ENO2.
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunofluorescence (1:500)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (10 mM sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot of ENO2 in rat spinal cord probed with ENO2 polyclonal antibodyCat # PAB12508 ). The antibody stains a single sharp band corresponing to NSE at about 47kDa.Immunofluorescence
The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to ENO2 using ENO2 polyclonal antibody (Cat # PAB12508). This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1. -
Gene Info — ENO2
Entrez GeneID
2026Gene Name
ENO2
Gene Alias
NSE
Gene Description
enolase 2 (gamma, neuronal)
Omim ID
131360Gene Ontology
HyperlinkGene Summary
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq
Other Designations
2-phospho-D-glycerate hydrolyase|enolase 2|neural enolase|neuron specific gamma enolase|neurone-specific enolase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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Publication Reference
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Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury.
Begaz T, Kyriacou DN, Segal J, Bazarian JJ.
Journal of Neurotrauma 2006 Aug; 23(8):1201.
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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
Shaw G, Morse S, Ararat M, Graham FL.
FASEB Journal 2002 Apr; 16(8):869.
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Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury.
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