Stat5a polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using Stat5a polyclonal antibody (Cat # PAB11328) shows detection of Stat5a protein in HeLa cell extract (arrowhead). Primary antibody was used at a 1 : 600 dilution reacted overnight at 4°C. Secondary antibody was used at 1 : 20,000. Molecular weight estimation was made by comparison to prestained MW markers. Identity of the upper and lower bands is unknown.
Personal communication, Luanne Lukes, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Stat5a.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Stat5a.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to mouse Stat5 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:25000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Stat5a polyclonal antibody (Cat # PAB11328) shows detection of Stat5a protein in HeLa cell extract (arrowhead). Primary antibody was used at a 1 : 600 dilution reacted overnight at 4°C. Secondary antibody was used at 1 : 20,000. Molecular weight estimation was made by comparison to prestained MW markers. Identity of the upper and lower bands is unknown.
Personal communication, Luanne Lukes, NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Stat5a
Entrez GeneID
20850Protein Accession#
NP_035618;P42230Gene Name
Stat5a
Gene Alias
AA959963, STAT5
Gene Description
signal transducer and activator of transcription 5A
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000002045|OTTMUSP00000002046|STAT5a variant delta E18|mammary gland factor STAT5A
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Publication Reference
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Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression.
Engblom D, Kornfeld JW, Schwake L, Tronche F, Reimann A, Beug H, Hennighausen L, Moriggl R, Schutz G.
Genes & Development 2007 May; 21(10):1157.
Application：ChIP, WB, Mouse, Liver.
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The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes.
Baugh JE Jr, Floyd ZE, Stephens JM.
Obesity (Silver Spring, Md.) 2007 Mar; 15(3):583.
Application：IP, WB, Mouse, 3T3-L1 cells.
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Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation.
Laurence A, Tato CM, Davidson TS, Kanno Y, Chen Z, Yao Z, Blank RB, Meylan F, Siegel R, Hennighausen L, Shevach EM, O'shea JJ.
Immunity 2007 Mar; 26(3):371.
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Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression.
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