Stat5a (phospho Y694) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using Stat5a (phospho Y694) polyclonal antibody (Cat # PAB11307) shows detection of phosphorylated Stat5a (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1 ku of IL-2 (lane 2).
No reactivity is seen for non-phosphorylated Stat5a in untreated cells (lane 1).
The primary antibody at a 1 : 1,000 dilution, overnight at 4°C.
Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Stat5a (phospho Y694) polyclonal antibody (Cat # PAB11307) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate positive staining of distal convoluted tubules and collecting ducts of human kidney at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Stat5a.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y694 of mouse Stat5a.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to mouse Stat5alpha protein phosphorylated at Y694.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:25000)
Western Blot (1:500-1:2000)
Immunohistochemistry (20-40 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Stat5a (phospho Y694) polyclonal antibody (Cat # PAB11307) shows detection of phosphorylated Stat5a (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1 ku of IL-2 (lane 2).
No reactivity is seen for non-phosphorylated Stat5a in untreated cells (lane 1).
The primary antibody at a 1 : 1,000 dilution, overnight at 4°C.
Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Stat5a (phospho Y694) polyclonal antibody (Cat # PAB11307) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate positive staining of distal convoluted tubules and collecting ducts of human kidney at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Stat5a
Entrez GeneID
20850Protein Accession#
NP_035618;P42230Gene Name
Stat5a
Gene Alias
AA959963, STAT5
Gene Description
signal transducer and activator of transcription 5A
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000002045|OTTMUSP00000002046|STAT5a variant delta E18|mammary gland factor STAT5A
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Publication Reference
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The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes.
Baugh JE Jr, Floyd ZE, Stephens JM.
Obesity (Silver Spring, Md.) 2007 Mar; 15(3):583.
Application：IP, WB, Mouse, 3T3-L1 cells.
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Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation.
Laurence A, Tato CM, Davidson TS, Kanno Y, Chen Z, Yao Z, Blank RB, Meylan F, Siegel R, Hennighausen L, Shevach EM, O'shea JJ.
Immunity 2007 Mar; 26(3):371.
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IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells.
Burchill MA, Yang J, Vogtenhuber C, Blazar BR, Farrar MA.
Journal of Immunology 2007 Jan; 178(1):280.
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The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes.
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