NFYA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol.
Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected to analysis by SDS-PAGE and immunoblot.
Using NFYA polyclonal antibody (Cat # PAB11297) at a 1 : 1000 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NFYA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NFYA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol.
Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected to analysis by SDS-PAGE and immunoblot.
Using NFYA polyclonal antibody (Cat # PAB11297) at a 1 : 1000 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFYA
Entrez GeneID
4800Gene Name
NFYA
Gene Alias
CBF-A, CBF-B, FLJ11236, HAP2, NF-YA
Gene Description
nuclear transcription factor Y, alpha
Omim ID
189903Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq
Other Designations
CAAT-box DNA binding protein subunit A|CCAAT-binding transcription factor subunit B|HAP2 CCAAT-binding protein|OTTHUMP00000016365|Transcription factor NF-Y, A subunit
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Interactome
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Pathway
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