NFYA rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human NFYA peptide using ARM Technology.
Immunogen
A synthetic peptide of human NFYA is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human NFYA peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — NFYA
Entrez GeneID
4800GeneBank Accession#
NFYAGene Name
NFYA
Gene Alias
CBF-A, CBF-B, FLJ11236, HAP2, NF-YA
Gene Description
nuclear transcription factor Y, alpha
Omim ID
189903Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq
Other Designations
CAAT-box DNA binding protein subunit A|CCAAT-binding transcription factor subunit B|HAP2 CCAAT-binding protein|OTTHUMP00000016365|Transcription factor NF-Y, A subunit
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Interactome
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Pathway
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