SLIT3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
Western blot using SLIT3 polyclonal antibody (Cat # PAB11260) shows detection ofa predominant band at ~145 KDa corresponding to SLIT3 (arrowhead) in a bovine thyroid tissue lysate using the 800 nm channel (green).
Approximately 35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 800.
Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-RabbitIgG [H&L] MXHu for 45 min atroom temperature.
Molecular weight markers were used for size comparison using the 700 nm channel (not shown).
IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye isa trademark of LI-COR, Inc.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SLIT3.
Immunogen
A synthetic peptide corresponding to amino acids 1164-1177 of human SLIT3.
Host
Rabbit
Reactivity
Bovine, Chicken, Chimpanzee, Dog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
Western blot using SLIT3 polyclonal antibody (Cat # PAB11260) shows detection ofa predominant band at ~145 KDa corresponding to SLIT3 (arrowhead) in a bovine thyroid tissue lysate using the 800 nm channel (green).
Approximately 35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 800.
Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-RabbitIgG [H&L] MXHu for 45 min atroom temperature.
Molecular weight markers were used for size comparison using the 700 nm channel (not shown).
IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye isa trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — SLIT3
Entrez GeneID
6586Protein Accession#
O75094;NP_003053Gene Name
SLIT3
Gene Alias
FLJ10764, MEGF5, SLIL2, SLIT1, Slit-3, slit2
Gene Description
slit homolog 3 (Drosophila)
Omim ID
603745Gene Ontology
HyperlinkOther Designations
slit homolog 3
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Rac1 inhibition via Srgap2 restrains inflammatory osteoclastogenesis and limits the clastokine, SLIT3.
Shin B, Kupferman J, Schmidt E, Polleux F, Delany AM, Lee SK.
Journal of Bone and Mineral Research 2019 Dec; [Epub].
Application：WB, Mouse, Mouse bone marrow monocytes.
-
Conserved modularity and potential for alternate splicing in mouse and human Slit genes.
Little M, Rumballe B, Georgas K, Yamada T, Teasdale RD.
The International Journal of Developmental Biology 2002 Jan; 46(4):385.
-
Dual trafficking of Slit3 to mitochondria and cell surface demonstrates novel localization for Slit protein.
Little MH, Wilkinson L, Brown DL, Piper M, Yamada T, Stow JL.
American Journal of Physiology. Cell Physiology 2001 Aug; 281(2):C486.
Application：IEM, IF, WB-Tr, Human, Monkey, Rat, COS-1, HeLa, M15 cells, Rat kidneys.
-
Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis.
Holmes GP, Negus K, Burridge L, Raman S, Algar E, Yamada T, Little MH.
Mechanisms of Development 1998 Dec; 79(1-2):57.
-
Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening.
Nakayama M, Nakajima D, Nagase T, Nomura N, Seki N, Ohara O.
Genomics 1998 Jul; 51(1):27.
-
Rac1 inhibition via Srgap2 restrains inflammatory osteoclastogenesis and limits the clastokine, SLIT3.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com