FOXO1 (phospho S319) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells untreated (Lane 1) or treated (Lane 2) with serum starvation using FFOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embbeded human breast carcinoma tissue using FFOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970) (A) or preincubated with blocking peptide (B).
Immunofluorescence
Immunofluorescence staining of manthanol-fixed HeLa cells using FOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of FOXO1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S319 of human FOXO1.
Host
Rabbit
Theoretical MW (kDa)
78-82
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells untreated (Lane 1) or treated (Lane 2) with serum starvation using FFOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embbeded human breast carcinoma tissue using FFOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970) (A) or preincubated with blocking peptide (B).Immunofluorescence
Immunofluorescence staining of manthanol-fixed HeLa cells using FOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970). -
Gene Info — FOXO1
Entrez GeneID
2308Protein Accession#
Q12778Gene Name
FOXO1
Gene Alias
FKH1, FKHR, FOXO1A
Gene Description
forkhead box O1
Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018301|forkhead box O1A (rhabdomyosarcoma)|forkhead homolog in rhabdomyosarcoma|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
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Interactome
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Pathway
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Disease
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