FOXO1 (phospho S256) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells untreated (Lane 1) or treated (Lane 2) with serum using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embbeded human breast carcinoma tissue using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969) (A) or preincubated with blocking peptide (B).
Immunofluorescence
Immunofluorescence staining of manthanol-fixed MCF-7 cells using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of FOXO1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S256 of human FOXO1.
Host
Rabbit
Theoretical MW (kDa)
78-82
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells untreated (Lane 1) or treated (Lane 2) with serum using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embbeded human breast carcinoma tissue using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969) (A) or preincubated with blocking peptide (B).Immunofluorescence
Immunofluorescence staining of manthanol-fixed MCF-7 cells using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969). -
Gene Info — FOXO1
Entrez GeneID
2308Protein Accession#
Q12778Gene Name
FOXO1
Gene Alias
FKH1, FKHR, FOXO1A
Gene Description
forkhead box O1
Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018301|forkhead box O1A (rhabdomyosarcoma)|forkhead homolog in rhabdomyosarcoma|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
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Interactome
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Pathway
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Disease
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Publication Reference
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Atypical PKC, PKCλ/ι, activates β-secretase and increases Aβ1-40/42 and phospho-tau in mouse brain and isolated neuronal cells, and may link hyperinsulinemia and other aPKC activators to development of pathological and memory abnormalities in Alzheimer's disease.
Sajan MP, Hansen BC, Higgs MG, Kahn CR, Braun U, Leitges M, Park CR, Diamond DM, Farese RV.
Neurobiology of Aging 2018 Jan; 61:225.
Application：WB-Ti, Mouse, Mouse brains.
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BMI-related progression of atypical PKC-dependent aberrations in insulin signaling through IRS-1, Akt, FoxO1 and PGC-1α in livers of obese and type 2 diabetic humans.
Sajan MP, Ivey III RA, Farese RV.
Metabolism 2015 Nov; 64(11):1454.
Application：WB-Ce, Human, Hepatocyte.
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Hepatic insulin resistance in ob/ob mice involves increases in ceramide, atypical PKC activity and selective impairment of Akt-dependent FoxO1 phosphorylation.
Sajan MP, Ivey RA, Lee MC, Farese RV.
Journal of Lipid Research 2015 Jan; 56(1):70.
Application：WB, Mouse, Liver, Muscle.
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PKCλ haplo-insufficiency prevents diabetes by a mechanism involving alterations in hepatic enzymes.
Sajan MP, Ivey RA 3rd, Lee M, Mastorides S, Jurzak MJ, Samuels VT, Shulman GI, Braun U, Leitges M, Farese RV.
Molecular Endocrinology 2014 Jul; 28(7):1097.
Application：WB-Ti, Mouse, Liver.
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Atypical PKC, PKCλ/ι, activates β-secretase and increases Aβ1-40/42 and phospho-tau in mouse brain and isolated neuronal cells, and may link hyperinsulinemia and other aPKC activators to development of pathological and memory abnormalities in Alzheimer's disease.
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