MBP polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Recombinant protein)
MBP polyclonal antibody (Cat # PAB10344) detects MBP-tagged recombinant proteins by western blot.
MBP epitope tag polyclonal antibody at 0.5-1.0 ug/mL was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag.
A 4-20% gradient gel was used to separate the protein by SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 2500 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
-
Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MBP (Maltose Binding Protein).
Immunogen
Recombinant protein corresponding to MBP (Maltose Binding Protein).
Host
Rabbit
Specificity
This polyclonal anti-MBP epitope tag antibody detects over-expressed proteins containing the MBP epitope tag.
Form
Lyophilized
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000-1:16000)
Western Blot (1:3500)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Recombinant protein)
MBP polyclonal antibody (Cat # PAB10344) detects MBP-tagged recombinant proteins by western blot.
MBP epitope tag polyclonal antibody at 0.5-1.0 ug/mL was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag.
A 4-20% gradient gel was used to separate the protein by SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 2500 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.Enzyme-linked Immunoabsorbent Assay
-
Publication Reference
-
Design of peptides as inhibitors of human papillomavirus 16 transcriptional regulator E1-E2.
Kantang W, Chunsrivirot S, Muangsin N, Poovorawan Y, Krusong K.
Chemical Biology & Drug Design 2016 Jun; 88(4):475.
Application：ELISA, WB, Recombinant protein.
-
Design of peptides as inhibitors of human papillomavirus 16 transcriptional regulator E1-E2.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com