RSL1D1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using RSL1D1 polyclonal antibody (Cat # PAB10306) shows detection of over-expressed RSL1D1 in lysates from HeLa cells transfected with Flag-RSL1D1.
Lanes 1 and 3 contain lysate from Flag-RSL1D1 transfected HeLa cells.
Lanes 2 and 4 contain lysate from cells transfected with null vector.
Lanes 1 and 2 were blotted with anti-Flag antibody.
Lanes 3 and 4 were probed with a 1 : 500 dilution of RSL1D1 polyclonal antibody.
The band at 75 KDa, indicated by the arrowhead, corresponds to RSL1D1.
Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Immunofluorescence
Immunofluorescence microscopy of HeLa cells transfected with RSL1D1 polyclonal antibody (Cat # PAB10306) .
In the overlay, specific antibody staining is shown to co-localize with recombinant protein.
Cells were fixed with methanol prior to staining.
Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RSL1D1.
Immunogen
A synthetic peptide corresponding to internal region of human RSL1D1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to human PBK1 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2500-1:10000)
Western Blot (1:500-1:2000)
Immunoprecipitation (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using RSL1D1 polyclonal antibody (Cat # PAB10306) shows detection of over-expressed RSL1D1 in lysates from HeLa cells transfected with Flag-RSL1D1.
Lanes 1 and 3 contain lysate from Flag-RSL1D1 transfected HeLa cells.
Lanes 2 and 4 contain lysate from cells transfected with null vector.
Lanes 1 and 2 were blotted with anti-Flag antibody.
Lanes 3 and 4 were probed with a 1 : 500 dilution of RSL1D1 polyclonal antibody.
The band at 75 KDa, indicated by the arrowhead, corresponds to RSL1D1.
Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.Immunofluorescence
Immunofluorescence microscopy of HeLa cells transfected with RSL1D1 polyclonal antibody (Cat # PAB10306) .
In the overlay, specific antibody staining is shown to co-localize with recombinant protein.
Cells were fixed with methanol prior to staining.
Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RSL1D1
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Interactome
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Disease
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Publication Reference
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Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells.
Tong C, Tan L, Li P, Zhu YS.
Acta Biochimica et Biophysica Sinica 2005 May; 37(5):303.
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Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.
Petroziello J, Yamane A, Westendorf L, Thompson M, McDonagh C, Cerveny C, Law CL, Wahl A, Carter P.
Oncogene 2004 Oct; 23(46):7734.
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Identification of differentially expressed genes in human trophoblast cells by differential-display RT-PCR.
Huch G, Hohn HP, Denker HW.
Placenta 1998 Nov; 19(8):557.
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Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells.
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