MFAP5 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using MFAP5 polyclonal antibody (Cat # PAB10261) shows detection (arrowhead) of secreted MFAP5 (lane 2) and MFAP5 present in a MFAP5 transfected HEK293 lysate (lane 3).
No staining is detected in supernatants from non-transfected cells (lane 1).
After SDS-PAGE and transfer the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4°C.
Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MFAP5.
Immunogen
A synthetic peptide corresponding to C-terminus of human MFAP5.
Host
Rabbit
Reactivity
Chimpanzee, Dog, Human, Mouse, Rat
Specificity
This affinity-purified antibody is directed against human MAGP-2 protein
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:250-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using MFAP5 polyclonal antibody (Cat # PAB10261) shows detection (arrowhead) of secreted MFAP5 (lane 2) and MFAP5 present in a MFAP5 transfected HEK293 lysate (lane 3).
No staining is detected in supernatants from non-transfected cells (lane 1).
After SDS-PAGE and transfer the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4°C.
Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MFAP5
Entrez GeneID
8076Protein Accession#
Q13361Gene Name
MFAP5
Gene Alias
MAGP2, MP25
Gene Description
microfibrillar associated protein 5
Omim ID
601103Gene Ontology
HyperlinkGene Summary
This gene encodes a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. It lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this encoded protein has some functions that are distinct from those of MFAP2. [provided by RefSeq
Other Designations
microfibril-associated glycoprotein 2|microfibril-associated glycoprotein-2
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Interactome
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Publication Reference
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The extracellular matrix protein MAGP-2 interacts with Jagged1 and induces its shedding from the cell surface.
Nehring LC, Miyamoto A, Hein PW, Weinmaster G, Shipley JM.
The Journal of Biological Chemistry 2005 May; 280(21):20349.
Application：IP, Monkey, COS-7 cells.
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The exon structure of the human MAGP-2 gene. Similarity with the MAGP-1 gene is confined to two exons encoding a cysteine-rich region.
Hatzinikolas G, Gibson MA.
The Journal of Biological Chemistry 1998 Nov; 273(45):29309.
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Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25).
Gibson MA, Hatzinikolas G, Kumaratilake JS, Sandberg LB, Nicholl JK, Sutherland GR, Cleary EG.
The Journal of Biological Chemistry 1996 Jan; 271(2):1096.
Application：IEM, IF, WB-Re, Bovine, Nuchal ligament tissues, Recombinant protein.
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The extracellular matrix protein MAGP-2 interacts with Jagged1 and induces its shedding from the cell surface.
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