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Western blot using GLI3 polyclonal antibody (Cat # PAB10052) shows detection of multiple bands in human lung lysate believed to be GLI3.
Lanes contain 20 ug of whole cell lysates from 1 - human brain, 2 - human lung, 3 - human spleen, 4 - mouse brain and 5 - mouse lung.
While no recognizable staining can be seen on mouse tissue, human lung shows what may be truncated GLI3 (~80 KDa).
This identity of the strong band at ~50 KDa is unknown.
After blocking, the membrane was probed with the primary antibody diluted to 1:500.
For detection use HRP Gt-a-Rabbit IgG.
Detection of GLI3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of GLI3 is likely to be low.
Furthermore, GLI3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Immunohistochemistry of GLI3 polyclonal antibody (Cat # PAB10052) was used at a 0.625 ug/mL to detect GLI3 in a variety of tissues.
Strong nuclear and smooth muscle staining was noted to be consistent with previously published reports.
Specific staining was noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus.
This image shows GLI3 staining of human glioblastoma.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.