CASP14 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of CASP14. Tissue lysates (50 ug/lane) and recombinant human CASP14 were (Hu C14, 15 ng) were western blotted with CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. The antisera detected both the proform of CASP14, and the large and small subunits of active/cleaved CASP14.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed paraffin-embedded sections from a human ovarian cancer tissue microarray stained for CASP14 expression. Using CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. Low (A) and high (B) stage ovarian tumor tissue cores. High magnification from areas of the tissue cores (A1 and B1) . Decreased CASP14 expression was seen in the high grade, compared to the low grade tumor. Hematoxylin-eosin counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed paraffin-embedded tissue sections of human cervix stained for CASP14 expression. Using CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. A. Normal cervix (squamous epithelium) . B. CIN1 (low-grade squamous intraepithelial lesion, mild dysplasia) . C. CIN2 (high-grade squamous intraepithelial lesion, moderate dysplasia) . D. CIN3 (high-grade squamous intraepithelial lesion ; severe dysplasia-carcinoma in situ) . In normal cervi, CASP14 staining was found most in the midzone layer, but was absent from the basal/parabasal cell layer where mitotically active cells are known to reside. This suggests induction of CASP14 expression with differentiation. CASP14 expression declined progressively during malignant transformation as the histologic severity of the cervical atypia advanced from CIN1 to CIN3. Hematoxylin-eosin counterstain.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant CASP14.
Immunogen
Recombinant protein corresponding to full length human CASP14.
Host
Rabbit
Reactivity
Dog, Gerbils, Human, Mouse, Rat
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of CASP14. Tissue lysates (50 ug/lane) and recombinant human CASP14 were (Hu C14, 15 ng) were western blotted with CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. The antisera detected both the proform of CASP14, and the large and small subunits of active/cleaved CASP14.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed paraffin-embedded sections from a human ovarian cancer tissue microarray stained for CASP14 expression. Using CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. Low (A) and high (B) stage ovarian tumor tissue cores. High magnification from areas of the tissue cores (A1 and B1) . Decreased CASP14 expression was seen in the high grade, compared to the low grade tumor. Hematoxylin-eosin counterstain.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed paraffin-embedded tissue sections of human cervix stained for CASP14 expression. Using CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. A. Normal cervix (squamous epithelium) . B. CIN1 (low-grade squamous intraepithelial lesion, mild dysplasia) . C. CIN2 (high-grade squamous intraepithelial lesion, moderate dysplasia) . D. CIN3 (high-grade squamous intraepithelial lesion ; severe dysplasia-carcinoma in situ) . In normal cervi, CASP14 staining was found most in the midzone layer, but was absent from the basal/parabasal cell layer where mitotically active cells are known to reside. This suggests induction of CASP14 expression with differentiation. CASP14 expression declined progressively during malignant transformation as the histologic severity of the cervical atypia advanced from CIN1 to CIN3. Hematoxylin-eosin counterstain.Immunoprecipitation
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Gene Info — CASP14
Entrez GeneID
23581Gene Name
CASP14
Gene Alias
MGC119078, MGC119079
Gene Description
caspase 14, apoptosis-related cysteine peptidase
Omim ID
605848Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq
Other Designations
apoptosis-related cysteine protease|caspase 14|caspase 14, apoptosis-related cysteine protease
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Interactome
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Disease
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Publication Reference
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Phospho-ΔNp63α regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation.
Ratovitski EA.
FEBS Letters 2013 Nov; 587(21):3581.
Application：WB-Tr, Human, SCC-11, SCC-11M, SCC-25, SCC-25CP cells.
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Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S.
Experimental Neurology 2004 Oct; 189(2):261.
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Phospho-ΔNp63α regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation.
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