IL10 (Human) Recombinant Protein
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Specification
Regulatory Status
RUO grade
Product Description
Human IL10 recombinant protein with His tag at the C-terminus expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 19.6
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 0.01 EU/ug
Activity
The ED50 for this effect is < 1 ng/mL, measured by its ability to induce MC/9‑2 cells proliferation. The specific activity of recombinant human IL-10 is approximately > 1 x 106 IU/mg.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 8.0.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 200 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — IL10
Entrez GeneID
3586Gene Name
IL10
Gene Alias
CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF
Gene Description
interleukin 10
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq
Other Designations
cytokine synthesis inhibitory factor
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