IL3 (Human) Recombinant Protein
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Specification
Regulatory Status
RUO grade
Product Description
Human IL3 recombinant protein with His tag at the C-terminus expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 16
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
The ED50 for this effect is < 0.15 ng/mL, measured by its ability to induce TF-1 cells proliferation. The specific activity of recombinant human IL-3 is approximately > 1.2 x 106 IU/mg.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 8.0.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 200 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — IL3
Entrez GeneID
3562Gene Name
IL3
Gene Alias
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
Gene Description
interleukin 3 (colony-stimulating factor, multiple)
Omim ID
147740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq
Other Designations
P-cell stimulating factor|hematopoietic growth factor|interleukin 3|mast-cell growth factor|multilineage-colony-stimulating factor
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