IL3 (Human) Recombinant Protein
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Specification
Product Description
Human IL3 (149 a.a.) full-lenth recombinant protein with His tag expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
Host
Plants
Theoretical MW (kDa)
20-23
Reactivity
Human
Form
Lyophilized
Preparation Method
Hordeum vulgare (barley) expression system
Purification
Chromatography
Purity
> 95% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kineticassay.
Activity
Activity has been measured by cell-based proliferation assay using TF-1 cells. ED50 is 0.18 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of IL3 (Human) Recombinant Protein (Cat # P5884) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — IL3
Entrez GeneID
3562Gene Name
IL3
Gene Alias
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
Gene Description
interleukin 3 (colony-stimulating factor, multiple)
Omim ID
147740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq
Other Designations
P-cell stimulating factor|hematopoietic growth factor|interleukin 3|mast-cell growth factor|multilineage-colony-stimulating factor
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