IL18BP (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-18 at 5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human IL-18BP, hFc Tag captured on Protein A chip, can bind Human IL-18 with an affinity constant of 0.04nM as determined in a SPR assay (Biacore T200).
Tris-Bis PAGE
Human IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human IL18BP (O95998-2, Thr31-Gly194) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Thr31-Gly194
Host
Human
Theoretical MW (kDa)
44.4
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 16.9 ng/mL, messured by ELISA at 5 ug/mL. The affinity constant of 0.04 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
Tris-Bis PAGE
Tris-Bis PAGE
Human IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-18 at 5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-18BP, hFc Tag captured on Protein A chip, can bind Human IL-18 with an affinity constant of 0.04nM as determined in a SPR assay (Biacore T200). -
Gene Info — IL18BP
Entrez GeneID
10068Protein Accession#
O95998-2Gene Name
IL18BP
Gene Alias
IL18BPa
Gene Description
interleukin 18 binding protein
Omim ID
604113Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. An elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
MC51L-53L-54L homolog gene product|OTTHUMP00000179141|OTTHUMP00000179143
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Disease
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