CRLF2 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human TSLPR, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 6.2 ng/mL determined by ELISA (QC Test).
SEC-HPLC
The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human TSLPR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human CRLF2 (Q9HC73-1, Gly25-Lys231) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly25-Lys231
Host
Human
Theoretical MW (kDa)
25.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 6.2 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human TSLPR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human TSLPR, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 6.2 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
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Gene Info — CRLF2
Entrez GeneID
64109Protein Accession#
Q9HC73-1Gene Name
CRLF2
Gene Alias
CRL2, CRLF2Y, TSLPR
Gene Description
cytokine receptor-like factor 2
Gene Ontology
HyperlinkGene Summary
Cytokine signals are mediated through specific receptor complexes, the components of which are mostly members of the type I cytokine receptor family. Type I cytokine receptors share conserved structural features in their extracellular domain. Receptor complexes are typically heterodimeric, consisting of alpha chains, which provide ligand specificity, and beta (or gamma) chains, which are required for the formation of high-affinity binding sites and signal transduction.[supplied by OMIM
Other Designations
cytokine receptor CRL2 precusor|thymic stromal lymphopoietin receptor|thymic stromal-derived lymphopoietin receptor
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Interactome
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Pathway
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Disease
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